AV短视频

Facult茅 de m茅decine Facult茅 de m茅decine

Research Groups

Developmental Genomics

fig._18_hopwoodcrop2.png Konrad Guenther, Vom Urtier zum Menschen, 1909.

During embryogenesis, the activation and repression of genes in time and in space instruct the fabric of organs and structures. Non-coding regions of the genome, called enhancers, orchestrate these activities and are therefore essential for organ development. Alterations in enhancer activities can lead to malformations or diseases.

Our laboratory investigates how enhancer repertoires shape normal and pathological gene expression domains to control organ patterning during embryogenesis. Our research currently focuses on three synergistic lines of study:

  • Generation of time- and cell-specific enhancers maps ()
  • Mechanisms underlying expression pattern formation ()
  • Mechanisms underlying pathogenic non-coding variations ()

Above: the expression domain (blue) of a patterning gene during limb development. Below: early- and late-acting enhancers function sequentially to establish the expression pattern of their cognate gene.

 

To address these aims, our lab, in collaboration with the institute's transgenesis facility uses tetraploid complementation, which enables to produce embryos from wildtype and genetically engineered Embryonic Stem Cells (ESCs). This approach that drastically limits the number of used animals was awarded the 2022 price. We then use cutting-edge methods to isolate cells of interest from these embryos, analyze transcriptional and chromatin alterations, and map enhancer regions.

Laboratory experimental workflow 

 

Selected Publications


Darbellay  F., Ramisch  A., Lopez-Delisle  L., Kosicki  M., Rauseo  A., Jouini  Z., Visel  A., Andrey G.
Nat Commun. 2024 Jun 6;15(1):4820. doi: 10.1038/s41467-024-49203-2.


Rouco R., Bompadre O., Rauseo A., Fazio O., Peraldi R., Thorel F. and Andrey G.
Nat Commun 12, 7235 (2021). https://doi.org/10.1038/s41467-021-27492-1


Paliou C., Guckelberger P., Schöpflin R., Heinrich V., Esposito A., Chiariello A.M., Bianco s., Annunziatella C., Helmuth J., Haas S., Jerkovi膰 I., Brieske N., Wittler L., Timmermann B., Nicodemi M., Vingron M., Mundlos S, Andrey G.
Proc Natl Acad Sci U S A. 2019 May 30. pii: 201900672. doi: 10.1073/pnas.1900672116


Kragesteen BK, Spielmann M, Paliou C, Heinrich V, Schöpflin R, Esposito A, Annunziatella C, Bianco S, Chiariello AM, Jerkovi膰 I, Harabula I, Guckelberger P, Pechstein M, Wittler L, Chan WL, Franke M, Lupiáñez DG, Kraft K, Timmermann B, Vingron M, Visel A, Nicodemi M, Mundlos S, Andrey G.
Nat Genet. 2018 Oct;50(10):1463-1473. doi: 10.1038/s41588-018-0221-x. Epub 2018 Sep 27.


Andrey G, Schöpflin R, Jerkovi膰 I, Heinrich V, Ibrahim DM, Paliou C, Hochradel M, Timmermann B, Haas S, Vingron M, Mundlos S.
Genome Res. 2017 Feb;27(2):223-233. doi: 10.1101/gr.213066.116. Epub 2016 Dec 6.


Franke M, Ibrahim DM, Andrey G, Schwarzer W, Heinrich V, Schöpflin R, Kraft K, Kempfer R, Jerkovi膰 I, Chan WL, Spielmann M, Timmermann B, Wittler L, Kurth I, Cambiaso P, Zuffardi O, Houge G, Lambie L, Brancati F, Pombo A, Vingron M, Spitz F, Mundlos S.
Nature. 2016 Oct 13;538(7624):265-269. doi: 10.1038/nature19800. Epub 2016 Oct 5.


Kraft K, Geuer S, Will AJ, Chan WL, Paliou C, Borschiwer M, Harabula I, Wittler L, Franke M, Ibrahim DM, Kragesteen BK, Spielmann M, Mundlos S, Lupiáñez DG, Andrey G.
Cell Rep. 2015 Feb 4. pii: S2211-1247(15)00029-7. doi: 10.1016/j.celrep.2015.01.016.


Andrey G, Montavon T, Mascrez B, Gonzalez F, Noordermeer D, Leleu M, Trono D, Spitz F, Duboule D.
Science. 2013 Jun 7;340(6137):1234167. doi: 10.1126/science.1234167.